Browsing by Author "Lubna Haleem"

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    Optimization Of Laboratory Requirements Through Experimental Design For The Production Of Indigenous Saccharomyces Cerevisiae From Orange Peels
    (J. Sc. & Tech. Univ. Peshawar, 2016) Faheem Ahmed Khan; Nafees Bacha; Sarzamin Khan; Lubna Haleem; Waqas Alam
    Orange peels are one of the main by-products of the citrus processing industry. Due to their microbial support capability on one hand, if discarded these may be a threat to a different form of life while on the other hand, they contain essential nutrients to support the growth of beneficial microbes for production of single cell microbial protein. For maximum microbial growth, the process of response surface methodology was utilized to optimize different parameters such as temperature, pH, shaking (revolutions per minute) and glucose concentration. It was observed that 2.36% glucose,10% substrate, 0.5% ammonium nitrate,1% yeast extract, pH 5,32oC temperature, and shaking at 150 rpm were best growth parameters for indigenous Saccharomyces cerevisiae and to obtain their proteins. Obtained single cell microbial protein was then evaluated for the presence of different amino acids. Results showed that concentration of aspartic acid was high followed by leucine i.e. 17.82±3.97% and 15.90±2.33% respectively on the other hand produced single cell protein was deficient in methionine. These results were analyzed through High-Performance Liquid Chromatography (HPLC) using Ortho Phthalaldehyde (OPA) as afluorescent agent. It is concluded that using response surface methodology different parameters can be effectively optimized for the growth of indigenous S. cerevisiae. As single cell microbial protein is the good source of amino acids these can be used as a protein source for human.