PHYTOCHEMICAL AND BIOASSAY SCREENING FOR FRUITS OF SARCOCOCCA SALIGNA (D.DON) MUEL AND AERIAL PARTS OF ASTRAGALUS ANISACANTHUS
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Center of Biotechnology
Abstract
Crude methanolic extract (Crd. MeOH Ext.) and different fractions ofS. saligna fruits
were investigated for various pharmacological / biological activities; Antimicrobial
(Antibacterial and Antifungal) Brine-shrimplethality, Phytotoxic, Heamagglutination,
Insecticidal, Anti-termites, Diphenyl Picryl Hydrazine (DPPH) free radical
scavenging assay, Acute toxicity, Analgesic, locomotr, Antidepressant and Gastro
Intestinal Tract Motility (GIT). These pharmacological activities revealed that the
plant possess good antibacterial, anti-termite, analgesic activity and GIT Motility.
Moderate phytotoxic activity was shown bythe test samples at higher concentration
(1000p:g/ml). Low antifungal, insecticidal, cytotoxic, locomotor and antidepressant
activity was shown by various fractions of this plant. A concentration dependent
DPPH free radical scavenging activity was shownby the test samples and moderate
ECso (58.61pg/ml). Fruits of the plant lack phytolectin as no heamagglutination
activity was observed against human erythrocytes. The S. saligna fruits extract
significantly inhibited the germination of wheat seeds (20g/72hrs). S. saligna fruits
showed the presence of carbohydrates, proteins, alkaloids, sterols and triterpenoids,
phenols, flavonoids, tannins and saponins. Elemental analysis showed the presence of
Sodium (Na), Magnesium (Mg), Zinc (Zn), Potassium (K), Nickel (Ni), Copper (Cu),
Lead (Pb), Manganese (Mn), Cobalt (Co), Cadmium (Cd), Iron (Fe) and Chromium
(Cr ). The proximat analysis showed carbohydrate, proteins, fibers, fats and moisture
content in fairly large amounts. The Scanning Electron Microscopy (SEM) technique
was used to determine the morphology ofthe S. saligna fruits powder. The Energy
Dispersive X-rays (EDX) technique was utilized to determine elemental composition.
Maximum number of oil component were identified through GC-MS analysis.