GREEN SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF ZINC AND COPPER NANOPARTICLES FROM AQUEOUS LEAVES EXTRACT OF ERIOBOTRYA JAPONICA AND FICUS CARICA
Loading...
Date
1012
Journal Title
Journal ISSN
Volume Title
Publisher
Center of Biotechnology
Abstract
The main aim of the study was to screen the methanolic, ethanolic and ethyl acetate
(EtOAc)fractions of Eriobotrya japonica and Ficus carica leaves and their Zinc (ZnNPs)
and Copper nanoparticles (CuNPs), respectively, for various pharmacological activities
such as, antibacterial, antifungal, phytotoxic, insecticidal, anti-termite, hemagglutination
and their comparison. The synthesis of ZnaNPs and CuNPs was carried out using aqueous leaf
extracts of E. japonica and F. carica, standardizing conditions for their synthesis like
temperature, pH and incubation. The characterization of these nanoparticles was done using
UV-Vis spectrophotometry, Fourier Transform Infrared spectroscopy, Scanning Electron
Microscope (SEM), Energy Dispersive X-ray (EDX), X-ray diffraction (XRD) and
Thermogravimetric/Differential Thermal Analysis (TGA/DTA). The UV-Visible absorption
spectra of ZnNPs and CuNPs showed peaks at 455 and 426 nm,respectively. The FTIR
spectra of ZnNPs and CuNPs confirmed the presence ofvarious functional groups suchas, -
hydroxyl, amide, carbonyl, aldehyde, and alkynes. The SEM analysis showed that the
particle size was between 99 and 96 nm with spherical shape. The presence of zinc and
copper along with other elements, such as C, O, S, Si, and K, was confirmed by EDX
analysis. The plant extract contributed the last four elements that stabilize and cap ZnNPsand
CuNPs. The mean size for ZnNPs and CuNPs in XRD spectra according to Debye-Scherer’s
equation was 33 and 32 nm,respectively. A clear antibacterial activity of the methanolic,
ethanolic and EtOAcfractions of E. japonica was observed in terms of percentinhibition:
E. coli (62, 59.2 and 44.4%), S. aureus (69.2, 46.1 and 50%), P. aeruginosa (44.4, 40.7 and
37%), M. morganii (39, 40.9 and 68 %), A. baumannii (52.1, 43.4 and 0 %), P. vulgaris
(60, 52 and 64%) and Methicillin Resistant Staphylococcus Aureus (MRSA)(73.6, 52.6
and 0 %). Similar results were also observed for methanolic, ethanolic and EtOAcfractions
of F. carica against E. coli (51.8, 40.7 and 48.8), S. aureus (65.3, 46.1 and 65.3), P.