MOLECULAR EPIDEMIOLOGY OF ANTIBIOTIC RESISTANT GENES IN PATHOGENIC BACTERIA, (A CASE STUDY OF KHYBER PUKIITUNKIIWA, PAKISTAI9
Date
2013
Journal Title
Journal ISSN
Volume Title
Publisher
Center of Biotechnology
Abstract
This study was conducted from 2009 to 2012 on isolates collected from two tedary
hospitals of Peshawar, I(iyber Pakhtunkhwa, Pakistan. The aim ofthis study was to find
out the resistance profile of different antibiotics and biocides, prevalence of extendedspectum
flactamases (ESBLs) and caxbapenemases ilt ,t aureus, E. coli, Klebsiella spp_
eJtd P. aeruginosa, rcspectively.
Among 133 S. aureus, 4'7yo werc Methicillin resistant S azreus (MRSA). Cefoxitin was
found to be the most efficient and effective method for the detection of MRSA. In S.
aureus, 'tal]lcomycir' (VAN) and linzolid (LZD) showed best activity against both MRSA
and MSSA. High level of antibiotic resista[ce was found in MRSA isolates. Most of our
,S. arrem isolates were susceptible to biocides. The genes including sea and pvl were the
most dominant genes in S. a,rezs isolates.
Carbapenems including meropenem and imipenem showed very good activity against E
coli and Klebsiella spp. and P aeruginosa. ESBL and carbapenem.tse prcducers were
also detected inE' coli arrd Klebsiella spp.
Results of the present study indicate that in Pakistan most of the infections are caused by
altibiotic resistarlt bacteda. The identification ofthese bacteria should be done properly.
Brilliance ESBL, CRE and MRSA agars were found best for the phenotypic detection of
Blactamases, caxbapenemases and MRSA.