GENETIC STUDIES OF OIL CONTENT IN MUTANT POPULATION OF RAPESEED (Brassica Napus L.) USING MOLECULAR MARKERS
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Date
2014
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Publisher
Center of Biotechnology & Microbiology, University of Peshawar
Abstract
The current study titled “genetic studies of oil content in mutant population of
rapeseed (Brassica napus L.) using molecular markers” was partially conducted at
Nuclear Institute for Food and Agriculture (NIFA), Peshawar in 2008-13. Main
objective of the study were to intimate and study the influence of induce mutation on
the genetics of oil and oil related components in advance induce mutant line of oilseed
rape (Brassica napus). Two contrast mutant lines with high and low oil contents were
crossed with each other and F1 hybrids were developed. These F1 hybrids were grown
in the cropping season 2009-10 and BCF1 were developed by crossing back the F1
with their parents. These BCF1 hybrids were grown in the next cropping season 2010-
11 and selfed to develop BCF2. The parent and the BCF2 hybrids were used for
molecular assessment to confirm mutation in the genetics of the advance mutant lines.
Thirty five advance mutant lines were utilized for agronomical and
biochemical study. Five plants were randomly selected from each mutant line and the
parent. Data on days to 50% flowering (DF), plant height (PH), 1000- seed weight
(SW), seed yield (SY) and oil yield (OY) were recorded. Seeds were analyzed at for
biomechanical parameter viz. Oil content (OC), protein content (PC), glucosinolates
(GSL), oleic acid (OA), linolenic acid (LA), and erucic acid (EA).