Bioinspired Synthesis, Characterization and Biological Evaluation of Silver Nanoparticles from aqueous leaves extract of hippophae rhamnoides linn
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Center of Biotechnology
Abstract
In this study, we have evaluated the physiochemical, minerals contents, phytochemicals, and biological activity of Hippophae rhamnoides Linn. (H. rhamnoides L.) leaves. The physiochemical and mineral contents of H. rhamnoides L. leaves revealed that fiber was 18.0 ± 2.6470, protein was 10.45 ± 0.88%, and carbohydrate value was found 68.75 ± 1%. Sodium was 30000 ± 1 ppm, calcium 7800 ± 1 ppm, and potassium 6200 ± 2 ppm as high concentration. While qualitative phytochemicals investigations showed that tannins, phenols, and flavonoids are present in large quantity. The Fourier transform infrared spectroscopy (FTIR) spectra of all extracts revealed the presence of different functional groups like O–H stretching for hydroxyl group, alkanes, alkenes, aromatic rings, carboxylic, and amides (aromatic).
The highest antibacterial zone of inhibition was observed in aqueous and methanolic extracts against S. aureus and V. cholerae with 21 ± 1 mm, and the lowest zone of inhibition measured 07 ± 0 mm against V. cholerae (ethyl acetate extracts). The antibacterial minimum inhibitory concentration values of extracts were determined ranging between 40 to 120 mg/ml, and minimum bactericidal concentration values of the extracts ranged between 50 and 135 mg/ml. The highest antifungal zone of inhibition was calculated against Alternaria alternata (18 ± 0 mm), followed by Aspergillus parasiticus (17 ± 1 mm) of methanolic extract, while Alternaria alternata (07 ± 1 mm) and Penicillium digitatum (07 ± 0 mm) were the least found in chloroform and aqueous extract, respectively. Further antifungal minimum inhibitory concentration and minimum fungicidal concentration are between 40–135 mg/ml and 50–180 mg/ml, respectively.