MOLECULAR EPIDEMIOLOGY OF STAPHYLOCOCCUS AUREUS, A CASE STUDY OF KHYBER PAKHTUNKHWA, PAKISTAN

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dc.contributor.authorFarman Ullah
dc.contributor.authorSupervised By : Prof. Dr. Bashir Ahmad
dc.date.accessioned2026-07-17T03:37:12Z
dc.date.available2026-07-17T03:37:12Z
dc.date.issued2015
dc.description.abstractThe present study was conducted from June 2010 to June 2014 in Khyber Teaching Hospital (KTH) and Hayatabad Medical Complex (HMC), Peshawar, Pakistan to know about the prevalence of resistance to antibiotics and molecular epidemiology of Staphylococcus aureus. The specimens include pus, blood and urine received for culture and sensitivity. S. aureus were identified at molecular level using polymerase chain reaction (PCR). The identified strains were subjected to antimicrobial testing by disc diffusion method and minimum inhibitory concentration (MIC) method according to the guide lines ofclinical laboratory and standards institute (CLSI). Cefoxitin, 30 pg discs were also applied in the culture sensitivity to find methicillin resistant S. aureus (MRSA) strains. Majority of S. aureus wereisolated from skin infections. A total of 273 (61.34%) isolates were found to be MRSA out of 445. The samples collected from KTH’s clinical laboratory were named as KLS (n=167), KTH’s environmental samples as KE (n=64), KTH’s blood isolates as KLBS (n=15), KTH’s currency notes as CRN (n=18), KTH’s burn ward samples as KBRN (n=89) and the isolates collected from Hayatabad medical complex were named as HMC (n=92). As a whole, amongtheantibiotics tested against S. aureus, vancomycin was most potent among alLantibiotics to which 89.89% isolates were susceptible. Next to vancomycin were fusidic acid, rifampicin, linezolid and chloramphenicol inhibiting 84.94, 83.37, 83.15 and 81.12 % of the isolates respectively. Among the 445 isolates, 73.03% were susceptible to doxycycline, 62.47 % to amikacin and 60% to meropenem. Gentamicin, clarithromycin and ciprofloxacin had average activity, ie. 53.93, 44.04 and 41.57 % respectively. Rest of the antibiotics had below 40 % activities. MRSA strains were found more resistant as compared to MSSA.
dc.identifier.urihttp://repository.uop.edu.pk/123456789/451
dc.language.isoen
dc.publisherCenter of Biotechnology
dc.relation.ispartofseries14730
dc.titleMOLECULAR EPIDEMIOLOGY OF STAPHYLOCOCCUS AUREUS, A CASE STUDY OF KHYBER PAKHTUNKHWA, PAKISTAN
dc.typeThesis
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