BIOTECHNOLOGICAL APPROACHES FOR IMPROVEMENT OF POTATO (SOLANUM TUBEROSUM L.)

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dc.contributor.authorMISS MUSSARAT JABEEN
dc.contributor.authorSupervised By: Prof. Dr. Farrukh Hussain
dc.date.accessioned2026-07-31T03:42:18Z
dc.date.available2026-07-31T03:42:18Z
dc.date.issued2013
dc.description.abstractShoot cultures were obtained by culturing the buds from disease free healthy potatoes of the cultivars Desiree and Ultimus, on both 4 and full strength Murashige and Skoog (MS)(1962) salt solution. Sprouting of the buds in both the cultivars was observed on the 3“ day of culture on 4 MS medium that grew into single leafy shoots, within 3 weeks, whereas on full MS medium sprouting of the buds in both the cultivars was delayed and occurred after the second week of culture. Moreover growth ofthe plantlets was also very slow on this medium. Nodal segments from these plantlets were then cultured on micropropagation media. The micropropagation medium consisted of % MS with 4% sucrose and supplemented with varying concentrations of NAA (0.1, 0.Smg/l), BAP (0.1, 0.5mg/1) GAS (0.2, 0.4, 0.6, 0.8mg/l) either alone or in combination. The experiments were carried out in two different day lengths (8 and 16hrs photoperiod). Best multiple shoot formation was observed on medium supplemented with 0.1mg/l NAA in combination with 0.Smg/1 BAP and 0.4mg/l GAin 16hrs photoperiod. The multiple shoots thus obtained were further subjected to different tuberization media. The tuberization media consisted of varying concentrations of different carbon sources (sucrose and glucose); different growth regulators such as BAP,Kinetin, Adenine sulphate (AS), IAA, Coumarin, ABA, GAs, Chlorocholine Chloride.
dc.identifier.urihttp://repository.uop.edu.pk/123456789/536
dc.language.isoen
dc.publisherDEPARTMENT OF BOTANY
dc.relation.ispartofseries100082
dc.titleBIOTECHNOLOGICAL APPROACHES FOR IMPROVEMENT OF POTATO (SOLANUM TUBEROSUM L.)
dc.typeThesis
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